identification of low molecular weight glutenin subunits in triticum aestivum and the d genome of wild related wheat

نویسندگان

سیوان احمدی

دانشجوی سابق کارشناسی ارشد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران محمدرضا نقوی

استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران علی اکبر شاه نجات بوشهری

استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; however, in ae. crassa, ʻbʼ and ʻcʼ alleles are the highest and lowest frequencies, respectively. the ʻaʼ and (ʻcʼ and ʻdʼ) alleles are the highest and lowest frequencies in ae. cylindrica; nonetheless, in ae. tauschii, ʻaʼ and ʻdʼ are the highest and lowest frequencies. in t. aestivum, ʻbʼ are the highest while ʻdʼ shows the lowest frequencies. genetic diversity (ζ) in populations of bread wheat, ae. crassa, ae. cylindrica, ae. tauschii and total population were calculated 0.6564, 0.5792, 0.6378, 0.6214 and 0.6294, respectively. furthermore, the average genetic diversity (h) for the total population was obtained 0.6294. the results show that wild wheat relatives have various alleles that can be utilized them in breeding programs to improve glutenin.

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